Guaifenesin stone matrix proteomics: a protocol for identifying proteins critical to stone formation.

ABSTRACT: Drug-related kidney stones are a diagnostic problem, since they contain a large matrix (protein) fraction and are frequently incorrectly identified as matrix stones. A urine proteomics study patient produced a guaifenesin stone during her participation, allowing us to both correctly diagnose her disease and identify proteins critical to this drug stone-forming process. The patient provided three random midday urine samples for proteomics studies; one of which contained stone-like sediment with two distinct fractions. These solids were characterized with optical microscopy and Fourier transform infrared spectroscopy. Immunoblotting and quantitative mass spectrometry were used to quantitatively identify the proteins in urine and stone matrix. Infrared spectroscopy showed that the sediment wa

SUBMITTER: Kolbach-Mandel AM 

PROVIDER: S-EPMC8496968 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature