Selective protein enrichment in calcium oxalate stone matrix: a window to pathogenesis?

ABSTRACT: Urine proteins are thought to control calcium oxalate stone formation, but over 1000 proteins have been reported in stone matrix obscuring their relative importance. Proteins critical to stone formation should be present at increased relative abundance in stone matrix compared to urine, so quantitative protein distribution data were obtained for stone matrix compared to prior urine proteome data. Matrix proteins were isolated from eight stones (> 90% calcium oxalate content) by crystal dissolution and further purified by ultradiafiltration (> 10 kDa membrane). Proteomic analyses were performed using label-free spectral counting tandem mass spectrometry, followed by stringent filtering. The average matrix proteome was compared to the average urine proteome observed in random urine samples f

SUBMITTER: Wesson JA 

PROVIDER: S-EPMC8496971 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature