Actovegin® reduces PMA-induced inflammation on human cells.

ABSTRACT:

Purpose

The effect of Actovegin® was investigated on PMA- and LPS-induced human peripheral blood mononuclear cells (PBMCs).

Methods

PBMCs (1 × 10⁶ cells/ml) from five blood donors (2 f, 3 m; 45-55 years) were grown in medium and exposed to Actovegin® in the presence or absence of PMA or LPS. Supernatants were collected to assess the concentration of cytokines (TNF-α, IL-1beta, IL-6 and IL-10). The reactive oxygen species (ROS) were assessed by a ROS-Glo^TM H₂O₂ assay.

Results

Stimulation of cells by PMA or LPS (without Actovegin®) significantly increased the secretion of IL-1beta, IL-6, IL-10 and TNF-α from PBMCs, compared to controls. Pre-treatment of cells with Actovegin® (1, 5, 25, 125 µg/ml) plus PMA significantly decreas

SUBMITTER: Reichl FX 

PROVIDER: S-EPMC8497287 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature