Mitochondrial and glycolytic extracellular flux analysis optimization for isolated pig intestinal epithelial cells.

ABSTRACT: Intestinal epithelial cells (IECs) are crucial to maintain intestinal function and the barrier against the outside world. To support their function they rely on energy production, and failure to produce enough energy can lead to IEC malfunction and thus decrease intestinal barrier function. However, IEC metabolic function is not often used as an outcome parameter in intervention studies, perhaps because of the lack of available methods. We therefore developed a method to isolate viable IECs, suitable to faithfully measure their metabolic function by determining extracellular glycolytic and mitochondrial flux. First, various methods were assessed to obtain viable IECs. We then adapted a previously in-house generated image-analysis algorithm to quantify the amount of seeded IECs. Correcting

SUBMITTER: Bekebrede AF 

PROVIDER: S-EPMC8497502 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature