High-resolution positron emission microscopy of patient-derived tumor organoids.

ABSTRACT: Tumor organoids offer new opportunities for translational cancer research, but unlike animal models, their broader use is hindered by the lack of clinically relevant imaging endpoints. Here, we present a positron-emission microscopy method for imaging clinical radiotracers in patient-derived tumor organoids with spatial resolution 100-fold better than clinical positron emission tomography (PET). Using this method, we quantify ¹⁸F-fluorodeoxyglucose influx to show that patient-derived tumor organoids recapitulate the glycolytic activity of the tumor of origin, and thus, could be used to predict therapeutic response in vitro. Similarly, we measure sodium-iodine symporter activity using ^99mTc- pertechnetate and find that the iodine uptake pathway is functionally conserve

SUBMITTER: Khan S 

PROVIDER: S-EPMC8497512 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature