Multi-Scale Light-Sheet Fluorescence Microscopy for Fast Whole Brain Imaging.

ABSTRACT: Whole-brain imaging has become an increasingly important approach to investigate neural structures, such as somata distribution, dendritic morphology, and axonal projection patterns. Different structures require whole-brain imaging at different resolutions. Thus, it is highly desirable to perform whole-brain imaging at multiple scales. Imaging a complete mammalian brain at synaptic resolution is especially challenging, as it requires continuous imaging from days to weeks because of the large number of voxels to sample, and it is difficult to acquire a constant quality of imaging because of light scattering during in toto imaging. Here, we reveal that light-sheet microscopy has a unique advantage over wide-field microscopy in multi-scale imaging because of its decoupling of illuminat

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8497830 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature