Global Profiling of the Lysine Crotonylome in Different Pluripotent States.

ABSTRACT: Pluripotent stem cells (PSCs) can be expanded in vitro in different culture conditions, resulting in a spectrum of cell states with distinct properties. Understanding how PSCs transition from one state to another, ultimately leading to lineage-specific differentiation, is important for developmental biology and regenerative medicine. Although there is significant information regarding gene expression changes controlling these transitions, less is known about post-translational modifications of proteins. Protein crotonylation is a newly discovered post-translational modification where lysine residues are modified with a crotonyl group. Here, we employed affinity purification of crotonylated peptides and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to systematically profile prot

SUBMITTER: Lv Y 

PROVIDER: S-EPMC8498919 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature