The [4Fe4S] Cluster of Yeast DNA Polymerase ε Is Redox Active and Can Undergo DNA-Mediated Signaling.

ABSTRACT: Many DNA replication and DNA repair enzymes have been found to carry [4Fe4S] clusters. The major leading strand polymerase, DNA polymerase ε (Pol ε) from Saccharomyces cerevisiae, was recently reported to have a [4Fe4S] cluster located within the catalytic domain of the largest subunit, Pol2. Here the redox characteristics of the [4Fe4S] cluster in the context of that domain, Pol2_CORE, are explored using DNA electrochemistry, and the effects of oxidation and rereduction on polymerase activity are examined. The exonuclease deficient variant D290A/E292A, Pol2_COREexo^-, was used to limit DNA degradation. While no redox signal is apparent for Pol2_COREexo^- on DNA-modified electrodes, a large cathodic signal centered at -140 mV vs NHE

SUBMITTER: Pinto MN 

PROVIDER: S-EPMC8499023 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature