Extracellular vesicles from maternal uterine cells exposed to risk factors cause fetal inflammatory response.

ABSTRACT:

Background

Fetal cell-derived exosomes (extracellular vesicles, 40-160 nm) are communication channels that can signal parturition by inducing inflammatory changes in maternal decidua and myometrium. Little is known about maternal cell-derived exosomes and their functional roles on the fetal side. This study isolated and characterized exosomes from decidual and myometrial cells grown under normal and inflammatory/oxidative stress conditions and determined their impact on fetal membrane cells.

Methods

Decidual and myometrial cells were grown under standard culture conditions (control) or exposed for 48 h to cigarette smoke extract or tumor necrosis factor-α, as proxies for oxidative stress and inflammation, respectively. Exosomes were isolated from media (differential ultra-ce

SUBMITTER: Shepherd MC 

PROVIDER: S-EPMC8499538 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature