Effect of docosahexaenoic acid on in vitro growth of bovine oocytes.

ABSTRACT:

Purpose

The present study investigated the effects of docosahexaenoic acid (DHA) on the growth of bovine oocytes.

Methods

Oocytes and granulosa cell complexes (OGCs) were collected from early antral follicles (0.4-0.7 mm) on the surface of ovaries harvested from a slaughterhouse. The OGCs were cultured with 0, 1, and 10 μmol/L docosahexanoic acid (DHA) for 16 days.

Results

Antrum formation of the OGCs and the number of granulosa cells (GCs) surrounding the oocytes were comparable among groups, whereas supplementation of 0.1 μmol/L of DHA significantly improved oocyte growth. Oocytes grown with DHA had a higher fertilization rate, acetylation levels of H4K12, and ATP contents, as well as a lower lipid content compared with those grown without DHA. In addition, GCs surr

SUBMITTER: Nagata S 

PROVIDER: S-EPMC8499585 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature