Functional and metabolic fitness of human CD4⁺ T lymphocytes during metabolic stress.

ABSTRACT: Human CD4⁺ T cells are essential mediators of immune responses. By altering the mitochondrial and metabolic states, we defined metabolic requirements of human CD4⁺ T cells for in vitro activation, expansion, and effector function. T-cell activation and proliferation were reduced by inhibiting oxidative phosphorylation, whereas early cytokine production was maintained by either OXPHOS or glycolytic activity. Glucose deprivation in the presence of mild mitochondrial stress markedly reduced all three T-cell functions, contrasting the exposure to resveratrol, an antioxidant and sirtuin-1 activator, which specifically inhibited cytokine production and T-cell proliferation, but not T-cell activation. Conditions that inhibited T-cell activation were associated with the down-

SUBMITTER: Holthaus L 

PROVIDER: S-EPMC8500231 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature