Inhibition of MAN2A1 Enhances the Immune Response to Anti-PD-L1 in Human Tumors.

ABSTRACT:

Purpose

Immune checkpoint blockade has shown remarkable efficacy, but in only a minority of patients with cancer, suggesting the need to develop additional treatment strategies. Aberrant glycosylation in tumors, resulting from the dysregulated expression of key enzymes in glycan biosynthesis, modulates the immune response. However, the role of glycan biosynthesis enzymes in antitumor immunity is poorly understood. We aimed to study the immunomodulatory effects of these enzymes.

Experimental design

We integrated transcriptional profiles of treatment-naïve human tumors and functional CRISPR screens to identify glycometabolism genes with immunomodulatory effects. We further validated our findings using in vitro coculture and in vivo syngeneic tumor growth assays.<

SUBMITTER: Shi S 

PROVIDER: S-EPMC8500537 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature