Substrate stiffness induced mechanotransduction regulates temporal evolution of human fetal neural progenitor cell phenotype, differentiation, and biomechanics.

ABSTRACT: While the mechanotransduction-induced fate of adult neural stem/progenitor cells (NPCs) is relatively known, how substrate stiffness regulates the temporal evolution of the biomechanics and phenotype of developmentally relevant human fetal NPCs (hNPCs) and their mechanosensing pathways remain unknown. Here, we primed hNPCs on tissue-culture plastic (TCPS) for 3 days in non-differentiating medium before transferring to TCPS or Geltrex™ gels (<1 kPa) for 9-day cultures post-priming, and regularly assessed stemness, differentiation, and cell mechanics (Young's modulus, tether forces, apparent membrane tension, tether radius). hNPCs maintained stemness on TCPS while those on gels co-expressed stemness and neural/glial markers, 3-days post-priming. Biomechanical characteristics remained unchang

SUBMITTER: Kothapalli C 

PROVIDER: S-EPMC8500671 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature