Construction of stable mouse artificial chromosome from native mouse chromosome 10 for generation of transchromosomic mice.

ABSTRACT: Mammalian artificial chromosomes derived from native chromosomes have been applied to biomedical research and development by generating cell sources and transchromosomic (Tc) animals. Human artificial chromosome (HAC) is a precedent chromosomal vector which achieved generation of valuable humanized animal models for fully human antibody production and human pharmacokinetics. While humanized Tc animals created by HAC vector have attained significant contributions, there was a potential issue to be addressed regarding stability in mouse tissues, especially highly proliferating hematopoietic cells. Mouse artificial chromosome (MAC) vectors derived from native mouse chromosome 11 demonstrated improved stability, and they were utilized for humanized Tc mouse production as a standard vector. In

SUBMITTER: Abe S 

PROVIDER: S-EPMC8501010 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature