Stage-resolved Hi-C analyses reveal meiotic chromosome organizational features influencing homolog alignment.

ABSTRACT: During meiosis, chromosomes exhibit dramatic changes in morphology and intranuclear positioning. How these changes influence homolog pairing, alignment, and recombination remain elusive. Using Hi-C, we systematically mapped 3D genome architecture throughout all meiotic prophase substages during mouse spermatogenesis. Our data uncover two major chromosome organizational features varying along the chromosome axis during early meiotic prophase, when homolog alignment occurs. First, transcriptionally active and inactive genomic regions form alternating domains consisting of shorter and longer chromatin loops, respectively. Second, the force-transmitting LINC complex promotes the alignment of ends of different chromosomes over a range of up to 20% of chromosome length. Both features correlate w

SUBMITTER: Zuo W 

PROVIDER: S-EPMC8501046 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature