Spatiotemporal evaporating droplet dynamics on fomites enhances long term bacterial pathogenesis.

ABSTRACT: Naturally drying bacterial droplets on inanimate surfaces representing fomites are the most consequential mode for transmitting infection through oro-fecal route. We provide a multiscale holistic approach to understand flow dynamics induced bacterial pattern formation on fomites leading to pathogenesis. The most virulent gut pathogen, Salmonella Typhimurium (STM), typically found in contaminated food and water, is used as model system in the current study. Evaporation-induced flow in sessile droplets facilitates the transport of STM, forming spatio-temporally varying bacterial deposition patterns based on droplet medium's nutrient scale. Mechanical and low moisture stress in the drying process reduced bacterial viability but interestingly induced hyper-proliferation of STM in macrophages,

SUBMITTER: Majee S 

PROVIDER: S-EPMC8501104 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature