Optimization of Metabolic Oligosaccharide Engineering with Ac₄GalNAlk and Ac₄GlcNAlk by an Engineered Pyrophosphorylase.

ABSTRACT: Metabolic oligosaccharide engineering (MOE) has fundamentally contributed to our understanding of protein glycosylation. Efficient MOE reagents are activated into nucleotide-sugars by cellular biosynthetic machineries, introduced into glycoproteins and traceable by bioorthogonal chemistry. Despite their widespread use, the metabolic fate of many MOE reagents is only beginning to be mapped. While metabolic interconnectivity can affect probe specificity, poor uptake by biosynthetic salvage pathways may impact probe sensitivity and trigger side reactions. Here, we use metabolic engineering to turn the weak alkyne-tagged MOE reagents Ac₄GalNAlk and Ac₄GlcNAlk into efficient chemical tools to probe protein glycosylation. We find that bypassing a metabolic bottleneck with a

SUBMITTER: Cioce A 

PROVIDER: S-EPMC8501146 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature