Transcriptome comparisons detect new genes associated with apoptosis of cattle and buffaloes preantral follicles.

ABSTRACT:

Background

To develop new breeding technology to improve the breeding ability of bovine, it is the development trend to find the main reason for the occurrence of atresia in these organisms. Transcriptomes of small (100-120 μm) and large (200-220 μm) preantral follicles from cattle and buffalo ovaries were evaluated in vivo and in vitro to understand the transcriptional modulation in preantral follicles that leads to the phenomenon of atresia.

Methods

The preantral follicles were checked as dead, damage, or live follicles in vivo and in vitro by using trypan blue then bisbenzimide and propidium iodine. Transcriptomes of small (100-120 μm) and large (200-220 μm) preantral follicles of cattle and buffalo were evaluated in vivo and in vitro by microarray and RT-PCR. Healthy pre

SUBMITTER: Zoheir KM 

PROVIDER: S-EPMC8501173 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature