Comparison of the frequencies of ENU-induced point mutations in male germ cells and inherited germline mutations in their offspring.

ABSTRACT:

Background

Gene mutations induced in germ cells may be transmitted to the next generation and cause adverse effects such as genetic diseases. Certain mutations may result in infertility or death in early development. Thus, the mutations may not be inheritable. However, the extent to which point mutations in male germ cells are transmitted to the next generation or eliminated during transmission is largely unknown. This study compared mutation frequencies (MFs) in sperm of N-ethyl-N-nitrosourea (ENU)-treated gpt delta mice and de novo MFs in the whole exome/genome of their offspring.

Results

Male gpt delta mice were treated with 10, 30, and 85 mg/kg of ENU (i.p., weekly × 2) and mated with untreated females to generate offspring. We previously reported a dose-dependent increa

SUBMITTER: Masumura K 

PROVIDER: S-EPMC8501628 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature