Biotinylation of the Neospora caninum parasitophorous vacuole reveals novel dense granule proteins.

ABSTRACT:

Background

Neospora caninum is an obligate intracellular parasite that invades host cells and replicates within the parasitophorous vacuole (PV), which resists fusion with host cell lysosomal compartments. To modify the PV, the parasite secretes an array of proteins, including dense granule proteins (GRAs). The vital role of GRAs in the Neospora life cycle cannot be overestimated. Despite this important role, only a subset of these proteins have been identified, and most of their functions have not been elucidated. Our previous study demonstrated that NcGRA17 is specifically targeted to the delimiting membrane of the parasitophorous vacuole membrane (PVM). In this study, we utilize proximity-dependent biotin identification (BioID) to identify novel components of the dense granules.

SUBMITTER: Yang C 

PROVIDER: S-EPMC8501707 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature