Voltage sensor movements of Ca_V1.1 during an action potential in skeletal muscle fibers.

ABSTRACT: The skeletal muscle L-type Ca²⁺ channel (Ca_V1.1) works primarily as a voltage sensor for skeletal muscle action potential (AP)-evoked Ca²⁺ release. Ca_V1.1 contains four distinct voltage-sensing domains (VSDs), yet the contribution of each VSD to AP-evoked Ca²⁺ release remains unknown. To investigate the role of VSDs in excitation-contraction coupling (ECC), we encoded cysteine substitutions on each S4 voltage-sensing segment of Ca_V1.1, expressed each construct via in vivo gene transfer electroporation, and used in cellulo AP fluorometry to track the movement of each Ca_V1.1 VSD in skeletal muscle fibers. We first provide electrical measurements of Ca_V1.1 voltage sensor charge movement in response

SUBMITTER: Banks Q 

PROVIDER: S-EPMC8501827 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature