Evidence for glutamine synthetase function in mouse spinal cord oligodendrocytes.

ABSTRACT: Glutamine synthetase (GS) is a key enzyme that metabolizes glutamate into glutamine. While GS is highly enriched in astrocytes, expression in other glial lineages has been noted. Using a combination of reporter mice and cell type-specific markers, we show that GS is expressed in myelinating oligodendrocytes (OL) but not oligodendrocyte progenitor cells of the mouse and human ventral spinal cord. To investigate the role of GS in mature OL, we used a conditional knockout (cKO) approach to selectively delete GS-encoding gene (Glul) in OL, which caused a significant decrease in glutamine levels on mouse spinal cord extracts. GS cKO mice (CNP-cre⁺ :Glul^fl/fl ) showed no differences in motor neuron numbers, size or axon density; OL differentiation and myelination in the ven

SUBMITTER: Ben Haim L 

PROVIDER: S-EPMC8502205 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature