Dataset Information

In-depth triacylglycerol profiling using MS³ Q-Trap mass spectrometry.

ABSTRACT: Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS³) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 - triacylglycerol profiling using MS³ - screens for more than 6,700 TAG species in a fully automated fashion. TriP-MS3 demonstrated excellent reproducibility (median interday CV ∼ 0.15) and linearity (median R² = 0.978) and detected 285 individual TAG species in human plasma. The semiquantitative accuracy of the method was validated by comparison with a state-of-the-art reverse phase liquid chromatography (RPLC)-MS (R² = 0.83), which is the most commonly used approach for TAGs profiling. Finally, we demonstrate the utility and the versatility of the method by characterizing the effects of a fatty acid desaturase inhibitor on TAG profiles in vitro and by profiling TAGs in Caenorhabditis elegans.

SUBMITTER: Cabruja M

PROVIDER: S-EPMC8502232 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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In-depth triacylglycerol profiling using MS<sup>3</sup> Q-Trap mass spectrometry.

Cabruja Matias M Priotti Josefina J Domizi Pablo P Papsdorf Katharina K Kroetz Deanna L DL Brunet Anne A Contrepois Kévin K Snyder Michael P MP

Analytica chimica acta 20210903

Total triacylglycerol (TAG) level is a key clinical marker of metabolic and cardiovascular diseases. However, the roles of individual TAGs have not been thoroughly explored in part due to their extreme structural complexity. We present a targeted mass spectrometry-based method combining multiple reaction monitoring (MRM) and multiple stage mass spectrometry (MS<sup>3</sup>) for the comprehensive qualitative and semiquantitative profiling of TAGs. This method referred as TriP-MS3 - triacylglycero ...[more]

PMID: 34625255

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Dataset Information

In-depth triacylglycerol profiling using MS³ Q-Trap mass spectrometry.

Publications

In-depth triacylglycerol profiling using MS<sup>3</sup> Q-Trap mass spectrometry.

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Dataset Information

In-depth triacylglycerol profiling using MS3 Q-Trap mass spectrometry.

Publications

In-depth triacylglycerol profiling using MS<sup>3</sup> Q-Trap mass spectrometry.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

In-depth triacylglycerol profiling using MS³ Q-Trap mass spectrometry.