BMP-treated human embryonic stem cells transcriptionally resemble amnion cells in the monkey embryo.

ABSTRACT: Human embryonic stem cells (hESCs) possess an immense potential to generate clinically relevant cell types and unveil mechanisms underlying early human development. However, using hESCs for discovery or translation requires accurately identifying differentiated cell types through comparison with their in vivo counterparts. Here, we set out to determine the identity of much debated BMP-treated hESCs by comparing their transcriptome to recently published single cell transcriptomic data from early human embryos ( Xiang et al., 2020). Our analyses reveal several discrepancies in the published human embryo dataset, including misclassification of putative amnion, intermediate and inner cell mass cells. These misclassifications primarily resulted from similarities in pseudogene expression, highli

SUBMITTER: Chhabra S 

PROVIDER: S-EPMC8502258 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature