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Generation of recombinant vaccinia virus and analysis of virus-induced cell death.

ABSTRACT: Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death regulators on virus-induced cell death. For complete details on the use and execution of this protocol, please refer to Liu et al. (2021).

SUBMITTER: Liu Z

PROVIDER: S-EPMC8503571 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Generation of recombinant vaccinia virus and analysis of virus-induced cell death.

Liu Zhijun Z Kang Kidong K Ka-Ming Chan Francis F

STAR protocols 20211004 4

Vaccinia virus is a large double-stranded DNA virus that is widely used to express foreign genes from different origins. We generated recombinant vaccinia virus that expresses a viral inhibitor to examine its effect on virus-induced necroptosis. We provide a detailed protocol to describe the generation of recombinant vaccinia virus, validation of protein expression, and determination of necroptosis using live cell imaging. This approach can be adapted to examine the effect of other cell death re ...[more]

PMID: 34661172

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