Rapid preparation of human blood plasma for bottom-up proteomics analysis.

ABSTRACT: This protocol offers step-by-step instructions for preparation of raw blood plasma for liquid chromatography - tandem mass spectrometry (LC-MS/MS) analysis in clinical proteomics studies. The technique is simple, robust, and reproducible, and the entire transformation from plasma proteins to desalted tryptic peptides takes only 3-4 h. The protocol ensures efficient denaturation of native proteases that, in combination with the speediness of the procedure, prevents non-specific and irreproducible cleavage of digested peptides. The protocol can be adopted for large-scale studies and automation. For complete details on the use and execution of this protocol, please refer to Overmyer et al. (2020).

SUBMITTER: Shishkova E 

PROVIDER: S-EPMC8503661 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature