Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.

ABSTRACT: The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions. However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration. We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration. ECM-enriched samples were isolated from planarians, and their proteomes were characterized by LC-MS/MS. The functions of identified ECM components were interrogated using RNA interference. Using this approach, we found that heparan s

SUBMITTER: Sonpho E 

PROVIDER: S-EPMC8503668 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature