Quantitative Measurement of Multiprotein Nanoparticle Interactions Using NMR Spectroscopy.

ABSTRACT: An effective intensity-based reference is a cornerstone for quantitative nuclear magnetic resonance (NMR) studies, as the molecular concentration is encoded in its signal. In theory, NMR is well suited for the measurement of competitive protein adsorption onto nanoparticle (NP) surfaces, but current referencing systems are not optimized for multidimensional experiments. Presented herein is a simple and novel referencing system using ¹⁵N tryptophan (Trp) as an external reference for ¹H-¹⁵N 2D NMR experiments. The referencing system is validated by the determination of the binding capacity of a single protein onto gold NPs. Then, the Trp reference is applied to protein mixtures, and signals from each protein are accurately quantified. All results are consiste

SUBMITTER: Xu JX 

PROVIDER: S-EPMC8504168 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature