Emergent Differential Organization of Airway Smooth Muscle Cells on Concave and Convex Tubular Surface.

ABSTRACT: Airway smooth muscle cells (ASMCs) exist in a form of helical winding bundles within the bronchial airway wall. Such tubular tissue provides cells with considerable curvature as a physical constraint, which is widely thought as an important determinant of cell behaviors. However, this process is difficult to mimic in the conventional planar cell culture system. Here, we report a method to develop chips with cell-scale tubular (concave and convex) surfaces from fused deposition modeling 3D printing to explore how ASMCs adapt to the cylindrical curvature for morphogenesis and function. Results showed that ASMCs self-organized into two distinctively different patterns of orientation on the concave and convex surfaces, eventually aligning either invariably perpendicular to the cylinder axis on

SUBMITTER: Jin Y 

PROVIDER: S-EPMC8505749 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature