Breakage of CRISPR/Cas9-Induced Chromosome Bridges in Mitotic Cells.

ABSTRACT: Chromosomal instability, the most frequent form of plasticity in cancer cells, often proceeds through the formation of chromosome bridges. Despite the importance of these bridges in tumor initiation and progression, debate remains over how and when they are resolved. In this study, we investigated the behavior and properties of chromosome bridges to gain insight into the potential mechanisms underlying bridge-induced genome instability. We report that bridges may break during mitosis or may remain unbroken until the next interphase. During mitosis, we frequently observed discontinuities in the bridging chromatin, and our results strongly suggest that a substantial fraction of chromosome bridges are broken during this stage of the cell cycle. This notion is supported by the observation that

SUBMITTER: Rodriguez-Munoz M 

PROVIDER: S-EPMC8505897 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature