Triptolide inhibits neutrophil extracellular trap formation.

ABSTRACT:

Background

Triptolide (PG490), as a triterpene dicyclic oxide has been reported to increase the generation of reactive oxygen species (ROS) and nitric oxide (NO) and induce apoptosis of RAW 264.7 cells in a dose-dependent manner. The activity of death NETs plays an important role in anti-bacterial processes in the human body. This study aimed to investigate the effect of triptolide (PG490) on neutrophil extracellular traps (NETs) formation.

Methods

After isolating peripheral blood neutrophils from healthy volunteers, cells were incubated with PG490 to observe and detect the level of NETs and detect the level of reactive oxygen species (ROS). The cells were cultured, stained and analyzed by fluorescence microscopy.

Results

Compared with the 12-myristate-13-acetate (PMA

SUBMITTER: Guan H 

PROVIDER: S-EPMC8506553 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature