RNA-binding protein RBM24 represses colorectal tumourigenesis by stabilising PTEN mRNA.

ABSTRACT:

Background

RNA-binding motif protein 24 (RBM24) functions as a splicing regulator, which is critical for organ development and is dysregulated in human cancers. Here, we aim to uncover the biological function of RBM24 in colorectal tumourigenesis.

Methods

Xenograft tumour model, Rbm24 knockout and Apc^min/+ mouse models were utilised. Colorectal cancer cells overexpressing or silencing RBM24 were established. RNA immunoprecipitation (RIP) assay was conducted to detect protein-RNA associations. Gene expression was measured by immunohistochemistry, western blotting, or quantitative PCR (qPCR).

Results

Rbm24-knockout mice developed spontaneous colorectal adenomas with lower expression of phosphatase and tensin homolog (PTEN). Immunohistochemical staining for th

SUBMITTER: Xia RM 

PROVIDER: S-EPMC8506628 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature