Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy.

ABSTRACT:

Background

Lysyl oxidase (LOX) has been identified to modulate osteoclast activity, so we explored the role of LOX_G473A, the highest frequency single nucleotide polymorphism in LOX, in osteoclast formation and its potential relationship to autophagy.

Methods

The ability of the LOX mutant, LOX_G473A, to promote autophagy and osteoclast formation was evaluated in the pre-osteoclast cell line RAW264.7. Furthermore, autophagy-related protein expression and autophagosomes were detected by western blot and electron microscopy, respectively. Simultaneously, osteoclast formation and resorption ability were also detected using TRAP staining assay and bone resorption assay. In addition, the osteoclast-related proteins and mRNAs, as well as p-AMPKα and p-mTOR prote

SUBMITTER: Zhang B 

PROVIDER: S-EPMC8506719 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature