T cell exhaustion drives osteosarcoma pathogenesis.

ABSTRACT:

Background

Osteosarcoma (OS) is a rare cancer with a bimodal age distribution that peaks in children and young adults. It has been shown that the expression of programmed cell death protein 1 (PD-1) and programmed death ligand-1 (PD-L1) on tumor-infiltrating immune cells negatively correlates with prognosis of OS patients. However, a comprehensive assessment of the tumor-infiltrating immune cells in OS and their function has not been performed.

Methods

CD8⁺ T cells were isolated from biopsy tissue samples collected from OS patients and control subjects. Mass cytometry, Treg suppression assay, mixed lymphocyte reaction assay, and effector T cell functional assay were performed to analyze the function of tumor-infiltrating T cells. A xenograft metastasis model was e

SUBMITTER: Sun CY 

PROVIDER: S-EPMC8506720 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature