Performance characteristics of the light-initiated chemiluminescent assay for quantitative determination of progesterone.

ABSTRACT:

Background

To study the performance of quantitative determination of progesterone by light-initiated chemiluminescent assay (LICA).

Methods

Clinical samples of serum were used for detection of progesterone by LICA. The precision study was performed according to Clinical and Laboratory Standards Institute (CLSI) EP15-A3, the linear range validation was performed according to CLSI EP06-A, accuracy was evaluated according to CLSI EP9-A3, and the performance of detection capability was confirmed according to CLSI EP17-A2. All data were analyzed using SPSS software. Function regression analysis was performed by OriginPro software.

Results

The LICA-800 system exhibited low coefficients of variation (CVs) and high reproducibility, and the calculated synthetic CV was 2.16%. T

SUBMITTER: Chen K 

PROVIDER: S-EPMC8506739 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature