Propofol protects hippocampal neurons in sleep-deprived rats by inhibiting mitophagy and autophagy.

ABSTRACT:

Background

Sleep deprivation (SD) causes a disturbance in the cognitive function of rats. While propofol has a powerful sedative and hypnotic effect and is an antioxidant, its effect on the cognitive function of rats following SD remains unknown. The purpose of this study was to explore the protective effects of propofol on excessive autophagy and mitophagy in the hippocampus of rats after SD.

Methods

Adult male rats were intraperitoneally injected with 30 mg/kg of propofol after 96 hours of SD. Then we evaluated the effect of propofol on the cognitive function of sleep deprived rats by the Morris water maze. Transmission electron microscopy, Western blotting, PCR, immunohistochemistry, autophagy enhancer and autophagy inhibitor were used to study the effect of propofol on h

SUBMITTER: Dai W 

PROVIDER: S-EPMC8506745 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature