Loss of miR-673-5p expression in the cornea promotes rat corneal allograft rejection by promoting Th17 cell differentiation mediated by JAK2/STAT3.

ABSTRACT:

Background

Cluster of differentiation 4 positive (CD4⁺) T cells play an important role in corneal graft rejection, especially the dynamic balance between regulatory T cells and helper T cells. This study aims to explore the upstream and downstream regulatory mechanisms of Th17 cell differentiation-mediated corneal allograft rejection.

Methods

By establishing rat corneal allograft transplantation model, transcriptome analysis was carried out to screen the differentially expressed genes related to T helper 17 (Th17) cell differentiation, and then cell experiments were used to verify the effect of miR-673-5p/Janus Kinase 2 (JAK2) signal on naïve CD4⁺ T cell differentiation and the proliferation, migration, and tube formation ability of human umbilical vein

SUBMITTER: Cao Q 

PROVIDER: S-EPMC8506749 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature