Profiles of messenger RNAs and MicroRNAs in hypoxia-induced hepatic stellate cells.

ABSTRACT:

Background

MicroRNA (miRNA) plays an important role in hepatic stellate cell (HSCs) activation and liver fibrosis. The purpose of this study is to explore the effect of hypoxia on the differential expression of mRNAs and miRNAs in rat HSCs.

Methods

HSC-T6 cells were treated with cobalt chloride (CoCl₂), and the activity of HSC-T6 cells was measured by the CCK-8 assay. The mRNA expression levels of hypoxia inducible factor-1α (HIF-1α), collagen type I, transforming growth factor-β1 (TGF-β1), and Smad7 were measured by RT-qPCR. The protein expression levels of HIF-1α, Bax, Bcl-2, and caspase-3 were assayed by western blot. We used basal medium and 400 µmol/L CoCl₂ medium to treat HSC-T6 cells for 48 h. Cells were harvested after 48 h to extract RNA. Trans

SUBMITTER: Zhang L 

PROVIDER: S-EPMC8506783 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature