Fraxetin attenuates ferroptosis in myocardial infarction via AKT/Nrf2/HO-1 signaling.

ABSTRACT:

Background

Myocardial infarction (MI) is the principal cause of mortality globally. Fraxetin (Fra) has anti-oxidative and anti-inflammatory properties. Nevertheless, the functional action of Fra in the progression of MI has never been elucidated.

Method

The in vivo model of MI was set up by ligating left anterior descending artery. The gene expression was tested by qRT-PCR and WB. The 2,3,5-triphenyltetrazolium chloride staining was applied to assess MI size. The cell viability was tested by MTT assay. Commercial kits were utilized to detect the activity of serum LDH and the levels of Fe²⁺, malondialdehyde (MDA), and glutathione (GSH).

Results

Fra treatment could reduce the infraction size and restrain ferroptosis in rats with MI. Moreover, Fra reduc

SUBMITTER: Xu Y 

PROVIDER: S-EPMC8507044 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature