Degradation of epigallocatechin and epicatechin gallates by a novel tannase Tan_Hcw from Herbaspirillum camelliae.

ABSTRACT:

Background

Herbaspirillum camelliae is a gram-negative endophyte isolated from the tea plant. Both strains WT00C and WT00F were found to hydrolyze epigallocatechin-3-gallate (EGCG) and epicatechin-3-gallate (ECG) to release gallic acid (GA) and display tannase activity. However, no tannase gene was annotated in the genome of H. camelliae WT00C.

Results

The 39 kDa protein, annotated as the prolyl oligopeptidase in the NCBI database, was finally identified as a novel tannase. Its gene was cloned, and the enzyme was expressed in E. coli and purified to homogeneity. Moreover, enzymatic characterizations of this novel tannase named Tan_Hcw were studied. Tan_Hcw was a secretary enzyme with a Sec/SPI signal peptide of 48 amino acids at the N-terminus, and it cat

SUBMITTER: Lei J 

PROVIDER: S-EPMC8507159 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature