FBXL20 promotes breast cancer malignancy by inhibiting apoptosis through degradation of PUMA and BAX.

ABSTRACT: Apoptosis is a programmed cell death that efficiently removes damaged cells to maintain tissue homeostasis. Defect in apoptotic machinery can lead to tumor development, progression, and resistance to chemotherapy. PUMA (p53 upregulated modulator of apoptosis) and BAX (BCL2 associated X protein) are among the most well-known inducers of apoptosis. It has been reported that expression levels of BAX and PUMA are controlled at the post-translational level by phosphorylation. However, the post-translational regulation of these pro-apoptotic proteins remains largely unexplored. In this study, using biochemical, molecular biology, flow cytometric, and immunohistochemistry techniques, we show that PUMA and BAX are the direct target of the F-box protein FBXL20, which restricts their cellular levels

SUBMITTER: Manne RK 

PROVIDER: S-EPMC8507197 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature