MiR-279-3p regulates deltamethrin resistance through CYP325BB1 in Culex pipiens pallens.

ABSTRACT:

Background

The overuse of insecticides to control insect vectors has promoted extensive insecticide resistance in mosquitoes. In this study, the functions of microRNA (miR)-279-3p and its target CYP325BB1 in the regulation of deltamethrin resistance in Culex pipiens pallens was investigated.

Methods

Quantitative real-time reverse transcription PCR was used to detect the expression levels of miR-279-3p and CYP325BB1. Then, the dual-luciferase reporter assay system, RNA interference, CDC bottle bioassay and Cell Counting Kit-8 (CCK-8) assay were used to explore the roles of these molecules in deltamethrin resistance both in vivo and in vitro.

Results

The expression patterns of miR-279-3p and CYP325BB1 were compared between deltamethrin-sensitive (DS-strain) and deltamet

SUBMITTER: Li X 

PROVIDER: S-EPMC8507342 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature