Comparing the Effect of Multiple Histone Deacetylase Inhibitors on SSTR2 Expression and [¹¹¹In]In-DOTATATE Uptake in NET Cells.

ABSTRACT: The aim of this study was to increase somatostatin type-2 receptor (SSTR2) expression on neuroendocrine tumor (NET) cells using histone deacetylase inhibitors (HDACis), potentially increasing the uptake of SSTR2-targeted radiopharmaceuticals and subsequently improving treatment efficacy of peptide receptor radionuclide therapy (PRRT). Human NET cell lines BON-1, NCI-H727, and GOT1 were treated with HDACis (i.e., CI-994, entinostat, LMK-235, mocetinostat, panobinostat, or valproic acid (VPA); entinostat and VPA were the HDACis tested in GOT1 cells) to examine SSTR2 mRNA expression levels and uptake of SSTR2-targeting radiotracer [¹¹¹In]In-DOTATATE. Reversibility of the induced effects was examined after drug-withdrawal. Finally, the effect of VPA on radiosensitivity was in

SUBMITTER: Klomp MJ 

PROVIDER: S-EPMC8508045 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature