Access to ultra-long IgG CDRH3 bovine antibody sequences using short read sequencing technology.

ABSTRACT: The advances in high-throughput DNA sequencing and recombinant antibody technologies has presented new methods for characterizing antibody repertoires and significantly increased our understanding on the functional role of antibodies in immunity and their use in diagnostics, vaccine antigen design and as biological therapeutics. A subset of Bos taurus antibodies possesses unique ultra-long third complementary-determining region of the heavy chain (CDRH3) and are of special interest because they are thought to have unique functional abilities of broadly neutralizing properties - a functional role that has not been fully explored in vaccine development. Next generation sequencing technologies that are widely used to profile immunoglobulin (Ig) repertoires are based on short-read methods such

SUBMITTER: Oyola SO 

PROVIDER: S-EPMC8508064 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature