Smoothelin-Like Protein 1 Regulates the Thyroid Hormone-Induced Homeostasis and Remodeling of C2C12 Cells via the Modulation of Myosin Phosphatase.

ABSTRACT: The pathological elevation of the active thyroid hormone (T₃) level results in the manifestation of hyperthyroidism, which is associated with alterations in the differentiation and contractile function of skeletal muscle (SKM). Myosin phosphatase (MP) is a major cellular regulator that hydrolyzes the phosphoserine of phosphorylated myosin II light chain. MP consists of an MYPT1/2 regulatory and a protein phosphatase 1 catalytic subunit. Smoothelin-like protein 1 (SMTNL1) is known to inhibit MP by directly binding to MP as well as by suppressing the expression of MYPT1 at the transcriptional level. Supraphysiological vs. physiological concentration of T₃ were applied on C2C12 myoblasts and differentiated myotubes in combination with the overexpression of SMTNL1 to asse

SUBMITTER: Major E 

PROVIDER: S-EPMC8508602 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature