Identification of Phosphorylated Calpain 3 in Rat Brain Mitochondria under mPTP Opening.

ABSTRACT: The protein phosphorylation of the membrane-bound mitochondrial proteins has become of interest from the point of view of its regulatory role of the function of the respiratory chain, opening of the mitochondrial permeability transition pore (mPTP), and initiation of apoptosis. Earlier, we noticed that upon phosphorylation of proteins in some proteins, the degree of their phosphorylation increases with the opening of mPTP. Two isoforms of myelin basic protein and cyclic nucleotide phosphodiesterase were identified in rat brain non-synaptic mitochondria and it was concluded that they are involved in mPTP regulation. In the present study, using the mass spectrometry method, the phosphorylated protein was identified as Calpain 3 in rat brain non-synaptic mitochondria. In the present study, th

SUBMITTER: Baburuna Y 

PROVIDER: S-EPMC8508669 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature