Selection and Validation of Candidate Reference Genes for Gene Expression Analysis by RT-qPCR in Rubus.

ABSTRACT: Due to the lack of effective and stable reference genes, studies on functional genes in Rubus, a genus of economically important small berry crops, have been greatly limited. To select the best internal reference genes of different types, we selected four representative cultivars of blackberry and raspberry (red raspberry, yellow raspberry, and black raspberry) as the research material and used RT-qPCR technology combined with three internal stability analysis software programs (geNorm, NormFinder, and BestKeeper) to analyze 12 candidate reference genes for the stability of their expression. The number of most suitable internal reference genes for different cultivars, tissues, and fruit developmental stages of Rubus was calculated by geNorm software to be two. Based on the results obtained

SUBMITTER: Wu Y 

PROVIDER: S-EPMC8508773 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature