Ultrafast Photoconversion Dynamics of the Knotless Phytochrome SynCph2.

ABSTRACT: The family of phytochrome photoreceptors contains proteins with different domain architectures and spectral properties. Knotless phytochromes are one of the three main subgroups classified by their distinct lack of the PAS domain in their photosensory core module, which is in contrast to the canonical PAS-GAF-PHY array. Despite intensive research on the ultrafast photodynamics of phytochromes, little is known about the primary kinetics in knotless phytochromes. Here, we present the ultrafast P_r ⇆ P_fr photodynamics of SynCph2, the best-known knotless phytochrome. Our results show that the excited state lifetime of P_r* (~200 ps) is similar to bacteriophytochromes, but much longer than in most canonical phytochromes. We assign the slow P_r* ki

SUBMITTER: Fischer T 

PROVIDER: S-EPMC8508867 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature