An Exon Skipping in CRS1 Is Associated with Perturbed Chloroplast Development in Maize.

ABSTRACT: Chloroplasts of higher plants are semi-autonomous organelles that perform photosynthesis and produce hormones and metabolites. They play crucial roles in plant growth and development. Although many seedling-lethal nuclear genes or regulators required for chloroplast development have been characterized, the understanding of chloroplast development is still limited. Using a genetic screen, we isolated a mutant named ell1, with etiolated leaves and a seedling-lethal phenotype. Analysis by BN-PAGE and transmission electron microscopy revealed drastic morphological defects of chloroplasts in ell1 mutants. Genetic mapping of the mutant gene revealed a single mutation (G-to-A) at the 5' splice site of intron 5 in CRS1, resulting in an exon skipping in CRS1, indicating that this muta

SUBMITTER: Wang M 

PROVIDER: S-EPMC8508894 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature