A Method to Monitor the NAD⁺ Metabolome-From Mechanistic to Clinical Applications.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD⁺) and its reduced form (NADH) are coenzymes employed in hundreds of metabolic reactions. NAD⁺ also serves as a substrate for enzymes such as sirtuins, poly(ADP-ribose) polymerases (PARPs) and ADP-ribosyl cyclases. Given the pivotal role of NAD(H) in health and disease, studying NAD⁺ metabolism has become essential to monitor genetic- and/or drug-induced perturbations related to metabolic status and diseases (such as ageing, cancer or obesity), and its possible therapies. Here, we present a strategy based on liquid chromatography-tandem mass spectrometry (LC-MS/MS), for the analysis of the NAD⁺ metabolome in biological samples. In this method, hydrophilic interaction chromatography (HILIC) was used to separat

SUBMITTER: Giner MP 

PROVIDER: S-EPMC8508997 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature